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Method of enzymatic assay for creatinine or of the ammonium ion; the assembly of the reagents for óoeuvre.

机译:肌酐或铵离子的酶促测定方法;成套试剂的组装。

摘要

The invention relates to a method of enzymatic assay for creatinine clearance, which consists in determining the quantity of ammonium ion released by creatinine clearance, characterized in that the determination is carried out in the presence of an acid hydroxamic or of one of its salts. & br / the invention also relates to a process for the elimination of errors in the enzymatic assay of the ammonium ion consisting in introducing into the reaction medium with the sample a hydroxamic acid or one of its salts. & br / the invention also relates to a set of reagents for the enzymatic assay for creatinine clearance, which comprises: & br / - of glutamate dehydrogenase (br / - creatinine iminohydrolase br / - of nadph br / - a salt of alpha - ketoglutaric br / - a solution of dilution br / - hydroxamic and an acid or one of its salts.
机译:本发明涉及用于肌酐清除率的酶促测定的方法,其在于确定由肌酐清除率释放的铵离子的量,其特征在于该测定在酸性异羟肟酸或其盐之一的存在下进行。 &本发明还涉及消除铵离子的酶法测定中的错误的方法,该方法包括将异羟肟酸或其盐之一与样品一起引入反应介质中。 &本发明还涉及用于肌酐清除率的酶促测定的一组试剂,其包括: br->-谷氨酸脱氢酶(br->-肌酸酐亚氨基水解酶--nadph-α-盐-酮戊二酸-稀释液-异羟肟酸和酸或其一种盐。

著录项

  • 公开/公告号FR2714676B1

    专利类型

  • 公开/公告日1996-02-23

    原文格式PDF

  • 申请/专利权人 PASTEUR SANOFI DIAGNOSTICS;

    申请/专利号FR19930015982

  • 发明设计人 SIMONE MARIO;

    申请日1993-12-31

  • 分类号C12Q1/32;C12Q1/34;

  • 国家 FR

  • 入库时间 2022-08-22 03:40:42

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