Method of detecting variant nucleic acids using extension oligonucleotides which differ from each other at both a position corresponding to the variant nucleic acids and one or more additional positions

摘要

Method of detecting nucleic acids whose nucleotide sequences differ from one another at a position X. The method uses a set of oligonucleotides where the nucleotides found at position X are complementary to a variant nucleic acids only at this position X. However, with respect to the position of another mismatch Y, the nucleotide sequences thereof differ from one another. The invention further addresses reagents for performing the methods as well as reagents and applications of the method.