The composition of nucleic acid enzymes multi-component (mnazima), methods for detecting the presence of at least one facilitator for combination, a target, a variant of the sequence of nucleic acids, for detecting the presence of at least one methylated nucleic acid and to detect sp Least a facilitator of combination using a cascade of expansion.Methods for fabricating a plurality of nucleic acid enzymes multi-component (mnazima), and the use of at least one oligonucleotu00ecdeo

摘要

The composition of nucleic acid enzymes multi-component (mnazima), methods for detecting the presence of at least one facilitator for combination, a target, a variant of the sequence of nucleic acids, for detecting the presence of at least one methylated nucleic acid, and to detect the A facilitator of combination using a cascade of expansion.Methods for fabricating a pluraridade enzymes multi-component nucleic acid (mnazima), and the use of at least one oligonucleotideo. The present invention relates to enzymes of nucleic acid muiticomponentes (mnazimas) and methods for their use.The mnazimas comprise two or more components oligonucleotideos that autocombinam in the presence of one or more molecules that facilitate the combination of mnazimas, to form a catalytically active structure. Methods are also provided for use mnazimas in detection, identification and / or quantification of one or more targets.The methods may be practiced in assays based in solution or in trials in which one or more components of the reaction are fastened to a support structure. The methods allow multiplexing detection of mnazimas to detect multiple targets in a single reaction. They are also provided to manufacture the kits, compositions, and to practice the methods provided here.