THE INTERACTION OF TB 3+ -PROTOCATECHUIC ACID COMPLEX WITH NUCLEIC ACIDS AND ITS APPLICATION IN DETERMINATION OF NUCLEIC ACIDS BASED ON FLUORESCENCE QUENCHING

摘要

@@INTRODUCTIONQuantitative determination of nucleic acids based on fluorescence and absorption properties is limited by low sensitivity and interferences1,2.Methods based on quenching or enhancement of light properties of a probe after interacting with nucleic acids have been developed based on luminescence rare earthions that have narrow spectral width,long luminescence lifetime,large Stokes shift and strong binding with biological molecules.O-Phenanthroline and pyridine arecommonly used as rare-earth ligands.