An isolated DNA Molecule encoding a Green Fluorescent Protein as a marker for traceable Plant Transformation, a method for production of transgenic plantsAN EXPRESSION vector, Transgenic plant cells and of these plants.

摘要

DNA molecules are provided for Transformation of cells in which the expression of a gene encoding the Protein Fluorescent verdeestu00e1 located under the control of an inducible Promoter.In addition, we provide DNA Molecules in which a Nucleotide sequence encoding Green Fluorescent laproteina,Is operatively linked to a sequence of Signal to swing porteina expressed uncompartimiento where no Subcellular Protein is toxic to the cell.Oxidative Stress in plant cells transformed with GFP can also improve mediantetransformaciu00f3n cells with an expression vector comprising genes that encode GFPPurification and Enzyme oxu00edgenotalcomo eru00f3xidodismutasa sup.The toxicity of GFP in plants transformadaspuede eliminated by removing the traceable Marker gene to cells or sectorestransformados.The FLP / FRT system is used in conjunction with GFP background I like marker for Transformation and excision of FRT.It also provides unamolu00e9cula nucleotide optimized for expression of a green fluorescent protein in plants.A method for the production of palntastransgu00e9nicas enel a vector P ortador a gene encoding the Green Fluorescent Protein into CellsThe cells are tracked to determine Protein are selected and transformed cells and regenerated.The toxicidadcelular of u00edna estu00e1circunscripta fluorescent protein expression by regulating the Gene that encodes the Protein or the direction of a Subcellular compartment where no ProteinEstu00f3xica to the cell. It also includes a Vector ofexpressing purchase of the DNA Molecule, a method to use such an expression vector