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Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
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机译:使用弱碱性聚合物捕获和选择性释放核酸的方法及其扩增
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摘要
Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with specific weakly basic polymers to form a precipitate with the nucleic acids at acidic pH. After removing non-precipitated materials, the pH is then made basic, thereby releasing the nucleic acids from the polymer. This method for preparing specimen samples is simple and quite rapid, and the released nucleic acids can be further treated in hybridization assays or amplification procedures. The weakly basic polymers are water-soluble and cationic at acidic pH, but neutral in charge at basic pH.
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