INCREASE OF PROTEIN EXPRESSION OF RECOMBINANT CYTOCHROME P450 ENZYMES BY CO-EXPRESSION WITH GENE ENCODING NADPH-P450 OXIDOREDUCTASE

摘要

A method for increasing protein expression of recombinant cytochrome P450 enzymes by co-expression with a gene encoding NADPH-P450 oxidoreductase is provided, which NADPH-P450 oxidoreductase oxidizes NADPH to obtain an electron and thus transfer the electron to the cytochrome P450 enzymes to help oxidation of foreign materials by the cytochrome P450 enzymes. The method for increasing protein expression of recombinant cytochrome P450 enzymes comprises co-expression of the recombinant cytochrome P450 enzyme genes with a gene encoding NADPH-P450 oxidoreductase, wherein the recombinant cytochrome P450 enzyme genes and the NADPH-P450 oxidoreductase gene are isolated from human or rat; the recombinant cytochrome P450 enzyme genes and the NADPH-P450 oxidoreductase gene are contained in one open reading frame; and the NADPH-P450 oxidoreductase induces increase of the protein amount of the recombinant cytochrome P450 enzymes expressed.