INCREASE OF PROTEIN EXPRESSION AND CATALYTIC ACTIVITY OF RECOMBINANT CYTOCHROME P450 ENZYMES BY CO-EXPRESSION WITH GENES ENCODING HEAT SHOCK PROTEINS

摘要

A method for increasing protein expression and catalytic activity of recombinant cytochrome P450 enzymes by co-expression with genes encoding heat shock proteins is provided, which heat shock proteins function as a molecular chaperone that regulates other protein synthesis and translocation, protein folding, gene expression, protein hydrolysis, protein aggregation, etc., so that the heat shock proteins induce increase of protein expression and catalytic activity of recombinant cytochrome P450 enzymes. The method for increasing protein expression and catalytic activity of recombinant cytochrome P450 enzymes comprises co-expression of the recombinant cytochrome P450 enzyme genes with genes encoding heat shock proteins Hsp70 and HDJ-1, wherein the recombinant cytochrome P450 enzyme genes and the heat shock protein genes are isolated from human or rat; the recombinant cytochrome P450 enzyme genes and the heat shock protein genes are contained in one open reading frame; and the heat shock proteins induce increase of the protein amount and catalytic activity of the recombinant cytochrome P450 enzymes.