DNA encoding a deoxyhypusine synthase plant, transgenic plants and a method for controlling senescence and programmed cell death in plants.

摘要

A method for inhibiting the expression of the endogenous deoxyhypusine synthase induced senescence in a plant, said method comprising (a) integrating into the genome of a plant a vector comprising (i) sequences substantially complementary antisense nucleotide to (1) a part corresponding to a chain of a molecule selected DNA from the group consisting of: (aa) a DNA molecule which hybridizes under high stringency conditions with SEQ ID NO: 1 and / or SEQ ID NO: 9 and which encodes a deoxyhypusine synthase; (Bb) a functional derivative of the DNA molecule of (aa) that hybridizes under high stringency to SEQ ID NO: 1 and / or SEQ ID NO: 9 and which encodes a deoxyhypusine synthase; (Cc) a DNA having the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 9; and (dd) a DNA molecule encoding the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 10, (2) a corresponding portion of one strand of a DNA molecule, wherein said DNA molecule hybridizes in high stringency conditions with SEQ ID NO: 5 encoding deoxyhypusine synthase endogenous senescence-induced, or (3) a corresponding portion of an RNA sequence encoded by a DNA molecule of (1) or (2); and (ii) regulatory sequences operatively linked to the antisense nucleotide sequences such that the antisense nucleotide sequences are expressed in a plant cell in which it is transformed; and (b) growing said plant, whereby said antisense nucleotide sequences are transcribed and bind to said RNA sequence, so that gene expression of endogenous senescence-induced deoxyhypusine synthase is inhibited.