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BIOLOGICAL METHODS OF ANALYSIS FOR QUALITY CONTROL OF NUTRICE WOMEN AND MEDICINES

机译:营养妇女和药品质量控制的生物学分析方法

摘要

1. A method for detecting or determining whether a substance has one or more useful biological, nutraceutical or therapeutic properties, which includes: performing expression in a sample of a first biological marker to suppress translation initiation from the first mRNA in the presence of a test substance: determining the expression level of said first biological marker in the specified sample; comparing the expression level of said first biological marker with a control expression level, wherein the expression level of said first biological marker exceeding a control expression level indicates that the test substance is a substance that has one or more favorable biological, nutraceutical or therapeutic properties. 2. The method of claim 1, wherein comparing the expression level of said first biological marker with a control expression level comprises: expressing in a sample a second biological marker to suppress translation initiation from a second mRNA in the presence of a control substance; determining an expression level of a second biological marker in a sample; comparing the expression level of said first biological marker with the expression level of said second biological marker. 3. The method according to any one of claims 1 or 2, wherein the first mRNA and the second mRNA have at least two open reading frames as part of their 5 'untranslated regions, and wherein the 5' untranslated region of the first mRNA is functionally connected to the coding region that encodes the specified first biological marker suppress
机译:1.一种检测或确定一种物质是否具有一种或多种有用的生物学,营养保健或治疗特性的方法,该方法包括:在存在测试的情况下,在样品中进行第一生物学标记的表达以抑制来自第一mRNA的翻译起始。物质:确定指定样品中所述第一生物标志物的表达水平;将所述第一生物标志物的表达水平与对照表达水平进行比较,其中所述第一生物标志物的表达水平超过对照表达水平表明所述测试物质是具有一种或多种有利的生物学,营养或治疗性质的物质。 2.根据权利要求1所述的方法,其中将所述第一生物标志物的表达水平与对照表达水平进行比较包括:在样品中表达第二生物标志物以在存在对照物质的情况下抑制来自第二mRNA的翻译起始;确定样品中第二生物标志物的表达水平;比较所述第一生物标志物的表达水平与所述第二生物标志物的表达水平。 3.根据权利要求1或2中任一项的方法,其中所述第一mRNA和第二mRNA具有至少两个开放阅读框作为其5'非翻译区的一部分,并且其中所述第一mRNA的5'非翻译区是功能上连接到编码指定的第一生物标记抑制物的编码区

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