Molecular breeding method of yeast having excellent stress tolerance and genetically modified yeast

摘要

PPROBLEM TO BE SOLVED: To provide a nucleic acid cassette and a method for producing gene-modified yeast having excellent stress resistance, and to provide the yeast. PSOLUTION: The nucleic acid cassette for producing the yeast having resistance to high sucrose stress or freezing stress, or both thereof includes a promotor induced by the high sucrose stress or the freezing stress, or both thereof; and a polynucleotide encoding ARO1 gene, BUD23 gene, PMR1 gene, SNF5 gene, GON7 gene, SPT20 gene, CBS2 gene or DBF2 gene of the yeast or the 5' terminal fragment thereof linked to the downstream side of the promoter so as to be active; or a combination thereof. PCOPYRIGHT: (C)2009,JPO&INPIT