Used to it quantitative analysis methods as well as to measure the amount of fluorescence using the surface plasmon excitation enhanced fluorescence spectroscopy quantitative analysis kit and analyte dissociation inhibitor

摘要

PROBLEM TO BE SOLVED: To provide a quantitative analysis method capable of inhibiting dissociation between a ligand and an analyte and performing a highly sensitive and precise detection with suppressed variation in obtained signals in the quantitative analysis method for measuring the fluorescence amount using surface plasmon field-enhanced fluorescence spectroscopy, a quantitative analysis kit used therefor, and an analyte dissociation inhibitor.;SOLUTION: A quantitative analysis method according to the present invention includes a specific sensor chip, nanostructured particles, and specific third ligands. The quantitative analysis method for an analyte by surface plasmon field-enhanced fluorescence spectroscopy uses a nanostructure formed by fixing a specific second ligand and the third ligand on the nanostructured particle. The quantitative analysis method includes performing predetermined processes.;COPYRIGHT: (C)2013,JPO&INPIT