QUANTITATIVE ANALYSIS METHOD FOR MEASURING FLUORESCENCE AMOUNT USING SURFACE PLASMON FIELD-ENHANCED FLUORESCENCE SPECTROSCOPY, QUANTITATIVE ANALYSIS KIT USED THEREFOR, AND ANALYTE DISSOCIATION INHIBITOR

摘要

PROBLEM TO BE SOLVED: To provide a quantitative analysis method capable of inhibiting dissociation between a ligand and an analyte and performing a highly sensitive and precise detection with suppressed variation in obtained signals in the quantitative analysis method for measuring the fluorescence amount using surface plasmon field-enhanced fluorescence spectroscopy, a quantitative analysis kit used therefor, and an analyte dissociation inhibitor.;SOLUTION: A quantitative analysis method according to the present invention includes a specific sensor chip, nanostructured particles, and specific third ligands. The quantitative analysis method for an analyte by surface plasmon field-enhanced fluorescence spectroscopy uses a nanostructure formed by fixing a specific second ligand and the third ligand on the nanostructured particle. The quantitative analysis method includes performing predetermined processes.;COPYRIGHT: (C)2013,JPO&INPIT