How to positioning in the collision-free micro-manipulation tools

摘要

This invention is the method of doing positioning for the sample carrier of the micro operation tool in collisionless making use of the microscope, focus position Z of the particular point P _P is calculated a) by adjusting the focus of the microscope object glass which possesses 1st numerical aperture NA₁ at least to one point P of the sample carrier surface, b) the micro operation tool is positioned on the optical axis of the microscope, focus position Z of c) micro operation tool _{M the} microscope object glass or the focus of the 2nd microscope object glass which smaller 2nd numerical aperture than as much as 1st numerical aperture NA₁ NA₂ possesses microIt seeks by adjusting to the point of the operation tool, d) considers specified allowance profile z and fall distance Z _A (P) it calculates Z _A (P) =Z with _{M Z P Z}, z= z _{M+ Z P} and z _M, z _P when calculating the respective operation tool or the position of the sample carrier, is specified allowance here, e) fall distance Z _A (just P) makes the micro operation tool fall and positions in the point P.