TUD RNA FOR KNOCKING DOWN MIRNA-29A, MIRNA-148A, AND MIRNA-424, AND APPLICATION THEREOF

摘要

Provided is a Tud RNA for knocking down miRNA-29a, miRNA-148a, and miRNA-424. A nucleotide sequence of the Tud RNA is represented by SEQ ID NO. 1. Also provided are a Tud RNA interference vector for suppressing expressions of miRNA-29a, miRNA-148a, and miRNA-424, and a preparation method therefor. The preparation method comprises: synthesizing, according to compared and analyzed human miR-29a, miR-148a, and miR-424 sequences, a fragment with BamHI and HindIII restriction enzyme cutting sites, connecting the fragment onto a plasmid vector subjected to BamHI and HindIII linearization, and filtering to obtain the interference vector.