TUD RNA FOR SUPPRESSING EXPRESSIONS OF MIRNA-29A, MIRNA-148A, AND MIRNA-152, AND APPLICATION THEREOF

摘要

Provided is a Tough Decoy RNA (Tud RNA) for suppressing expressions of miRNA-29a, miRNA-148a, and miRNA-152. A nucleotide sequence of the Tud RNA is represented by SEQ ID NO. 1. The present invention also provides a Tud RNA interference vector for suppressing expressions of miRNA-29a, miRNA-148a, and miRNA-152. A preparation method for the Tud RNA interference vector comprises: synthesizing, according to compared and analyzed human miR-29a, miR-148a, and miR-152 sequences, a fragment with BamHI and HindIII restriction enzyme cutting sites, connecting the fragment onto a plasmid vector subjected to BamHI and HindIII linearization, and filtering to obtain an effective interference vector.