Methods for generation, identification and isolation of human pluripotent stem cell-derived cardiomyocytes and cardiomyocyte subpopulations

摘要

The present invention provides a method for the generation, isolation, detection and / or analysis of cardiomyocytes derived from a starting cell composition comprising pluripotent and / or multipotent stem cells, comprising: a) pluripotency and And / or differentiating multipotent stem cells into cardiovascular cells, thereby producing a sample comprising cardiomyocytes and non-cardiomyocytes; and b) the non-cardiomyocytes of the sample are specific for non-cardiomyocyte antigens Labeling with multiple antibodies or antigen binding fragments thereof, and c) depleting labeled non-cardiomyocytes from the sample; and optionally d) cardiomyocytes of the sample specific for cardiomyocyte antigens Labeling with at least one antibody or antigen-binding fragment thereof; and e) cardiomyocyte suspension derived from pluripotent and / or multipotent stem cells, wherein the labeled cardiomyocytes are concentrated The method comprising the steps of detecting and isolating type.