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PROCESS FOR GENERATION, IDENTIFICATION AND ISOLATION OF HUMAN PLURIPOTENT STEM CELL-DERIVED CARDIOMYOCYTES AND CARDIOMYOCYTE SUBPOPULATIONS
PROCESS FOR GENERATION, IDENTIFICATION AND ISOLATION OF HUMAN PLURIPOTENT STEM CELL-DERIVED CARDIOMYOCYTES AND CARDIOMYOCYTE SUBPOPULATIONS
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机译:生成,鉴定和分离人类多能干细胞衍生心肌细胞和心肌细胞群的过程
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摘要
The present invention provides a method for generation, isolation, detection and/or analysis of cardiomyocytes derived from a starting cell composition comprising pluripotent and/or multipotent stem cells, the method comprising the steps a) differentiating said pluripotent and/or multipotent stem cells into cardiovascular cells, thereby generating a sample comprising cardiomyocytes and non-cardiomyocytes; and b) labeling the non-cardiomyocytes of said sample with more than one antibody or antigen binding fragment thereof specific for antigens of non-cardiomyocytes and c) depleting said labeled non-cardiomyocytes from said sample; and optionally d) labeling the cardiomyocytes of said sample with at least one antibody or antigen binding fragment thereof specific for antigen(s) of cardiomyocytes; and e) enriching said labeled cardiomyocytes and detecting and isolating cardiomyocyte subtypes derived from said pluripotent and/or multipotent stem cells.
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