miRNA miRNA classifier for for the diagnosis of lymph node metastasis of colorectal cancer and a method for diagnosis using the same as

摘要

The present invention provides a miR-342-3p, miR-3621, and a 3-miRNA classifier of miR361-3p as a miRNA discriminator for diagnosing lymph node metastasis of colorectal cancer. Of the 3-miRNA classifiers identified in the present invention, miR-342-3p and miR-361-3p are down-regulated in LNM positive CRC compared to LNM negative, and miR-3621 is positive for lymph node metastasis. Lymph node metastasis is up-regulated compared to negative in CRC, which can be used as a biomarker for determining lymph node metastasis in colorectal cancer. In addition, E2F1, RAP2B, FOXM1, PIK3R3, AKT1, and WINT5A, which are proteins expressed from the target genes of miR-342-3p, miR-361-3p, or miR-3621, also significantly increased lymphocytic expression in CRCs. In addition, it can be used as a biomarker for determining lymph node metastasis in colorectal cancer. When the protein expressed from the 3-miRNA fractionator of the present invention and its target gene is used as a biomarker, it is possible to more accurately and quickly determine whether lymph node metastasis has occurred in early colorectal cancer, so that if the endoscopic ablation is performed. It can be expected that the number of unnecessary additional surgeries can be reduced for a possible T1-phase CRC, which is effective.