METHOD OF PRODUCING A MAMMALIAN CELL CULTURE MEDIA COMPRISING HIGH MOLECULAR WEIGHT RECOMBINANT VON WILLEBRAND FACTOR (rVWF)

摘要

The claimed invention relates to a method of producing a mammalian cell culture media comprising high molecular weight recombinant Von Willebrand Factor (rVWF) in the presence of 2.4-20 g/L of copper salt, 4 mM of ammonium, 2.510⁶ cells per mL. The invention also relates to a mammalian cell culture media produced by the claimed method having an rVWF specific ristocetin cofactor activity of at least 30 mU/g rVWF.