method for producing a recombinant von willebrand factor composition (rvwf), cell culture supernatant, and recombinant von willebrand factor composition (rvwf)

摘要

method for producing a recombinant von willebrand factor composition (rvwf), cell culture supernatant, and recombinant von willebrand factor composition (rvwf). among other aspects, the present invention relates to cell culture conditions for the production of high molecular weight vwf, in particular highly multimeric wf with a high specific activity and adamts13 with a high specific activity. The cell culture conditions of the present invention may include, for example, a cell culture medium with a higher concentration of copper and / or cell culture supernatant with a concentration of copper and / or low culture cell supernatant. of ammonium (nh4). The present invention also provides methods for culturing cells under cell culture conditions for expressing high molecular weight vwf and rvwf having high specific activities.