METHOD FOR SCREENING COMPOUND BY MEANS OF HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY COMBINED WITH BIOLUMINESCENCE METHOD

摘要

A method for screening a compound by means of a high-performance thin-layer chromatography combined with a bioluminescence method, relating to the technical field of food detection. The high-performance thin-layer chromatography and the bioluminescence method are combined for use. The method comprises: spreading a sample to be detected on a thin-layer plate, then coupling to a luminescent bacterium, performing luminescence imaging, and finally performing scanning by using the thin-layer chromatography. The method for screening the compound by means of the high-performance thin-layer chromatography combined with the bioluminescence method can be used for detecting multiple samples, however, only one sample can be detected by using a high-performance liquid chromatography (HPLC). Compared with an existing HPLC, the present invention can achieve fast screening, simultaneously detect multiple kinds of samples in a single experiment, and achieve high throughput screening; a detection limit can reach 0.5-1 mg/kg; the detection method can achieve the repeatability less than 3.4%, and is economic, fast, simple and convenient.