A Validated High-Performance Thin-Layer Chromatography (Hptlc) Method for the Quantitative Determination of Tricin in Two iSpergularia/iSpecies

摘要

A simple, sensitive, precise and robust high-performance thin-layer chromatographic (HPTLC) method for analysis of tricin has been developed and validated for the determination of tricin in genus Spergularia. Analysis of tricin was performed on pre-coated TLC aluminum plates with silica gel as the stationary phase. Linear ascending development was carried out in double twin trough glass chamber saturated with a mobile phase consisting of chloroform: methanol (37:3 v/v). Spectro-densitometric scanning was performed by TLC scanner III (CAMAG) in absorbance mode at the wavelength of 270 nm. The system was found to give compact spots for tricin (Rf value of 0.34 ± 0.04). The linear regression analysis data for the calibration plots showed a good linear relationship with r2 = 0.9981 in the concentration range 2-15 μl with respect to peak area. According to the International Conference on Harmonization (ICH) guidelines, the method was validated for precision, recovery, and robustness. The tricin content quantified from Spergularia marina and S. daiandra was found to be 0.398 mg/100g and 1.642 mg/100g fresh weight, respectively. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of tricin.