Primary replacement for the detection of 8bp deletion mutation in Exon 3 of the 21-hydroxylase (CYP 21) gene and its procedures

摘要

Primary replacement for the detection of an 8bp deletion mutation in Exon 3 of the 21-hydroxylase gene, which allows the diagnosis of congenital adrenal hyperplasia (Congenital Adrenal Hyperplasia CAH), having a forward (F)-Primer that is long 21 base pairs, and a backward (R)-Primer that is long 19 base pairs, with the sequence:(i) (F) CAGACCACT(ii) (R) CAGACING AGREEMENT.