Contractile effect of Sclerocarya birrea (A Rich) Hochst (Anacardiaceae) (Marula) leaf aqueous extract on rat and rabbit isolated vascular smooth muscles

摘要

Background: Sclerocarya birrea (Anacardiaceae) is traditionally used for treating hypertension. The pharmacological effects of S birrea leaf aqueous extract (SBE) on rabbit and rat vascular smooth muscles were investigated in this study. Methods: Fresh S birrea leaves (1 kg) were air dried at 26 ± 1°C, milled, macerated in 2.5 l of distilled water for 48 hours, filtered, and the filtrate was concentrated in a rotary evaporator. Rat isolated portal vein preparations, as well as rabbit isolated endothelium-denuded and endothelium-intact descending thoracic aortic ring preparations were mounted in 30-ml Ugo Basile organ baths under physiological conditions, and challenged with SBE (50-400 mg/ml). The contractile effects of SBE and/or other reference drugs on the isolated vascular smooth muscle preparations were recorded by means of Ugo Basile's force-displacement transducers and Gemini recorders. Results: SBE (50-400 mg/ml) caused a significant, concentration-dependent upward shift in baseline tone in the aortic ring preparations (p 0.01-0.001). Indomethacin (20 μM) markedly attenuated the contractile effects of SBE in both the endothelium-intact and -denuded aortic rings, while N G-nitro- L-arginine methyl ester (L-NAME, 100 μM) significantly (p 0.05) increased the contractile tension of the endothelium-intact aortic rings. Verapamil (1-3 μg/ml) partially inhibited the contractile effects of SBE. SBE also elicited significant (p 0.05-0.01) increases in the amplitude of the myogenic contractions of the portal veins. These contractions were abolished by verapamil (1-3 μg/ml) in a concentration-dependent manner, while prazosin (1-3 μg/ml) did not affect the SBE-induced contractions. Conclusion: SBE possessed spasmogenic effects on vascular smooth muscle preparations in vitro. It may induce and/or exacerbate hypertension, contrary to the folkloric, ethnomedical use of S birrea.