Analytical study of charge transfer complexation ofrabeprazole with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone

摘要

A simple, sensitive spectrophotometric method was developed for the determination of rabeprazole sodium(RA) in pure form and for pharmaceutical formulations. The charge transfer interaction between RA and 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) as an acceptor was investigated spectrophotometrically. Variables affecting the reactionwere studied and optimized. The proposed method was applied successfully to the determination of RA in its pure form andpharmaceutical dosage forms with good accuracy and precision. The formation of charge transfer complex and the site ofinteraction was confirmed by using UV-Visible spectrophotometry, FT-IR spectrometry, and ~1H NMRtechniques. Based onJob's method of continuous variation plots, the obtained results indicate the formation of 1:1 charge transfer complex with ageneral formula [(RA) (DDQ)]. Statistical comparison of the results with the reference method shows excellent agreementand indicates no significant differences in accuracy or precision.