Chemical imaging of nucleic acids, proteins and lipids of a single living cell. Application of synchrotron infrared microspectrometry in cell biology.

摘要

Hybridoma B-cells have been used as models to evaluate the performance of synchrotron infrared microscopy to obtain chemical images of a single living cell. Chemical mapping of nucleic acids, proteins and lipids at a resolution of a few microns, close to the diffraction limit in the mid-infrared region are shown.