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首页> 外文期刊>Livestock Science >Electron microscopy finder-grids link quantitative cytometry with high-resolution confocal imaging in the study of TGF- beta 1 expression in the small intestine mucosa in growing pigs.
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Electron microscopy finder-grids link quantitative cytometry with high-resolution confocal imaging in the study of TGF- beta 1 expression in the small intestine mucosa in growing pigs.

机译:电子显微镜寻像器网格将定量细胞计数法与高分辨率共聚焦成像相结合,用于研究生长猪小肠粘膜中TGF-β1的表达。

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摘要

A new method is proposed linking the high-throughput quantitative population analysis with high-resolution imaging systems. Samples from piglet small intestine labelled against transforming growth factor beta 1 (TGF- beta 1) were overlayed with electron microscopy finder-grids and scanned by SCAN^R scanning cytometer. From the tissue map generated by SCAN^R, regions of interest (ROI) with TGF- beta 1-positive cells were selected and their coordinates stored from the sample overview. ROI were then located under the confocal microscope enabling detail visualization of the pattern of intracellular localization of the studied cytokine.
机译:提出了一种将高通量定量种群分析与高分辨率成像系统联系起来的新方法。将标记有转化生长因子β1(TGF-β1)的仔猪小肠的样品用电子显微镜查找器栅格覆盖,并通过SCAN ^ R扫描细胞仪进行扫描。从SCAN ^ R生成的组织图中,选择具有TGF-β1阳性细胞的感兴趣区域(ROI),并从样品概览中存储其坐标。然后将ROI置于共聚焦显微镜下,从而能够详细显示所研究细胞因子的细胞内定位模式。

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