Production of alternan by Leuconostoc mesenteroides CBI-110

摘要

Alternan known as a kind of dextran is a linear and cyclic polysaccharide which is synthesized with sucrose by alternansucrase (EC 2.4.1.140) of Leuconostoc mesenteroides. But this polysaccharide is different from dextran in its structure and other properties. For the purpose of applying alternan for the food, cosmetic, and pharmaceutical industries, we isolated an excellent alternan-producing strain, Leu. mesenteroides CBI-110. The optimum culture conditions were studied to improve the yield, and the activity of alternansucrase was increased up to 33U/mL in the optimum culture media. The concentrations of Mn~(2+) and Cu~(2+) ions were 0.01% and 0.03%, respectively, for the effective production of alternan. Finally, we obtained 25.6 g/L of alternan.