Characterization of a mosquitocidal delat-endotoxin from Bacillus thruingiensis subsp. guiyangiensis strain 21-2(H serotype 43)

摘要

To prevent appearance of resistant mosquitoes against #delta#-endotoxin of Bacillus thuringiensis subsp, israelensis (Bti) in field, a mosquitocidal Bacillus thuringiensis strain 21-2 (Bt 21-2) producing a new type of #delta#-endotoxin was isolated. The strain At 21-2 belongs to H serotype 43, B. thuringiensis subsp, guiyangiensis (Btg). The #delta#-endotoxins from the strain Bt 21-2 and the strain Bti were a cuboid shape morphologically, but the #delta#-endotoxin from the type strain Btg was a bipyramidal shape on phase contrast microscope. The #delta#-endotoxin of the strain Bt 21-2 was composed of 150, 90 and 70 KDa proteins on SDS-PAGE, and the antigenicity of #delta#- endotoxin of the strain Bt 21-2 was different from that of the strain Bti on immunoblot. The #delta#-endotoxin gene of the strain Bt 21-2 was not amplified with specific primers of #delta#-endotoxin gene (cry4A and cry4B) of the strain Bti on PCR.