Studies on the response of Photobacterium phosphoreum to the volatile substances

摘要

Various materials including sodium alginate, k-carrageenan, collagen and polyacrylamide were studied in order to maintain the stability of bioluminescence of Photobacterium phosphoreum for the monitoring of volatile toxic substances. Kinetic parameters of specific rate(p.), and gamma(y) value were determined for the relationship between bioluminescence of immobilized P phosphoreum and toxic substances. The bioluminescence intensity was found to be proportional to the concentration of toxic substances and the free cells were shown to be more sensitive than immobilized cells when volatile substances were exposed to the cells. Bioluminescence increased slightly after several minutes, which was due to the volatility of toxic compounds. Furthermore. P phosphoreum immobilized on strontium alginate was better than cells immobilized on sodium alginate for the response to substances used.