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首页> 外文期刊>Mitochondrial DNA, Part A >The complete mitochondrial genome of the southern purple-spotted gudgeon Mogurnda adspersa (Perciformes: Eleotridae) through pyrosequencing
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The complete mitochondrial genome of the southern purple-spotted gudgeon Mogurnda adspersa (Perciformes: Eleotridae) through pyrosequencing

机译:通过焦磷酸测序的南部紫色斑点g鱼Mogurnda adspersa(Perciformes:Eleotridae)的完整线粒体基因组

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The complete mitochondrial genome of the Critically Endangered southern purple-spotted gudgeon, Mogurnda adspersa, was determined for the first time using 1/8 of a 454 pyrosequencing plate. The mitogenome was assembled using the bioinformatic softwareMIRA. The M. adspersa genome organization was very similar to most vertebrates, being 16,523 bp in length. It contained 13 protein-coding genes, 22 transfer RNA, 2 ribosomal RNA genes, and 1 non-coding control region. The current methodology was effective to assemble the whole mitogenome using 2008 mitochondrial reads representing 0.23% of total genomic reads produced and providing average mitogenome coverage of 17.4 reads per site. The whole mitogenome sequence provided here is deposited in NCBI withthe accession number KJ130031 and may benefit systematics studies and conservation programs of M. adspersa.
机译:使用1/8的454焦磷酸测序板,首次确定了极度濒危的南部紫色斑点g鱼Mogurnda adspersa的完整线粒体基因组。使用生物信息学软件MIRA组装有丝分裂基因组。 adspersa分枝杆菌的基因组组织与大多数脊椎动物非常相似,长度为16,523 bp。它包含13个蛋白质编码基因,22个转移RNA,2个核糖体RNA基因和1个非编码控制区。当前的方法有效地组装了整个有丝分裂基因组,使用的是2008年线粒体读段,占生产的总基因组读段的0.23%,每个站点的平均有丝分裂基因组覆盖率为17.4个读段。此处提供的完整有丝分裂基因组序列已保藏在NCBI中,登录号为KJ130031,可能有益于M. adspersa的系统研究和保护程序。

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