Comparison of immunohistochemistry and Ziehl-Neelsen for the detection of Mycobacterium infection in sea bass (Dicentrarchus labrax).

摘要

Ziehl-Neelsen (ZN) and immunohistochemistry were used to detect Mycobacteria in histological sections of internal organs from sea bass (Dicentrarchus labrax) intraperitoneally inoculated with Mycobacterium fortuitum. Both methods allowed for detection of advanced lesions caused by Mycobacteria, but immunohistochemistry allowed for their detection in early granulomas. Thus immunohistochemistry is suited to the study of Mycobacterium pathogenesis in fish and may improve the diagnostic capacity for these bacteria..