Multiple Shoot and Callus Induction of Herbaceous Peony (Paeonia lactiflora Pall.)

摘要

Underground buds of herbaceous peony (Paeonia lactiflora Pall.) 'Zhong Sheng Fen' were used as explants for axillary shoot induction while stems, petioles and leaves of 'Da Fu Gui' and 'Tao Hua Fei Xue' were used as explants for callus induction. Theeffects of different basal media and concentrations of plant growth regulators (PGRs) on induction were investigated to establish an aseptic regeneration system. The best medium to induce and proliferate shoots was modified half-strength Murashige and Skoog (MS) medium with double the concentration of Ca~(2+) supplemented with 1 mg l~(-1) gibberellic acid (GA_3) plus 1 mg l~(-1) 6-benzyladenine (BA). Two successive steps were adopted for rooting shoots. Shoots were first cultured on Woody Plant Medium (WPM) plus 0.5 mg l~(-1) 1-naphthyleneacetic acid (NAA) for 10-15 days in the dark, then shoots were transferred to PGR-free WPM medium containing 0.2% activated charcoal; in this case, rooting could reach 50%. The best explants for callus induction wereyoung stems, and the best basal medium for callus induction was WPM for 'Da Fu Gui' and 'Tao Hua Fei Xue'.