DNA PROFILING OF A FEW INDIGENOUS AND EVOLVED SILKWORM BREEDS OF INDIA USING MICROSATELLITE MARKERS

摘要

DNA profiling of 15 Indian silkworm breeds comprising of 10 newly evolved and 5 indigenous polyvoltine breeds was carried out using 12 sets of microsatellite primers. Analysis of the DNA banding pattern has shown varying DNA profile with most of the microsatellite primers. For these 12 SSR loci, a total of 87 alleles were detected in the 15 silkworm breeds. The number of alleles detected at each locus in these breeds varied from two alleles to as high as fifteen. The heterozygosity values for the SSR alleles ranged from 0.251 to 0.933 and among the 15 silkworm breeds, the similarity index values varied from 0.435 to 0.792. Clustering based on microsatellite profiles, resulted in reasonable groupings of indigenous and evolved silkworm breeds. Breed specific microsatellite alleles have been detected, indicating the prospects of using microsatellite markers for establishment of molecular IDs for distinguishing silkworm breeds.