METABOLIC CHANGES DURING EMBRYOGENESIS IN NON-DIAPAUSE, ARTIFICIALLY DIAPAUSE TERMINATED AND. DIAPAUSE EGGS OF SILKWORM,BOMBYXMORIL.

摘要

The study of nucleic acids and glycogen level during embryogenesis would appear to be a more accurate way of defining metabolic activity during course of organogenesis. A specific pattern of variation in the metabolism of nucleic acids, and glycogen was noticed in non -diapause and diapause eggs due to the significant differences in embryonic development. There was a gradual increase in RNA titer during embryogenesis in non-diapause (ND), acid treated diapause eggs (AT) and reached a peak on the dayof hatching. While, in diapause eggs, the level was not consistent, but after 40"1 day it raised till 90>k day of diapause development. The DNA level was found to be significantly higher in AT-diapause eggs compared to ND eggs at the day of hatching, though it was very low at Ohr of egg laying. A steep rise in glycogen content was initially observed at 18 hrs of egg laying in ND and AT eggs and thereafter it content lowered in all the cases. But in diapause eggs, no significant changes was observed in glycogen titer from day 10 to day 60, followed by a significant fall at day 70 to reach a very minimum level at day 90 after oviposition. Though drastic changes were observed in content of biomolecules, the metabolic rate was found to be accelerated in non- diapause eggs just after egg deposition, while it was very slow in diapause eggs. When the diapause eggs were treated with HCL within 16-20 hrs, the rate of turnover was found to very similar to non-diapause eggs, though the base level of RNA, DNA andglycogen was recorded to be different.