Use of Diapausing Eggs from Killifish for Rapid, Sensitive Biological Screeningof Toxicants in the Aquatic Environment. Phase II

摘要

A 108-tank recirculating system was constructed for the commercial breeding andmaintenance of a large adult population of annual killifish, Nothobranchius guentheri. Embryos were harvested and shipped to collaborating USABRDL for use in preliminary toxicant assessment studies. Several studies investigating the effects of temperature on Diapause 3 termination and hatching were conducted. Results permitted the development of long term storage techniques for embryos to be used in portable toxicant assessment kits. Results support the use of annual killifish eggs in a portable rapid toxicant assessment kit with a 90 day or greater shelf life.