NCM460, A NORMAL HUMAN COLON MUCOSAL EPITHELIAL CELL LINE

摘要

Many studies of human intestinal physiology and pathology would be augmented with the development of normal human cell lines (1,2). These include investigations of transport, drug delivery, infectious disease, and other processes. Developing normal human cell lines is particularly important for colon cancer where malignant cell lines (8) have dominated the in vitro studies. This has been true because of the lack of easily propagated normal cell lines, even though it has been well-recognized from detailed genetic analyses of in situ tissues (10) that genetic and other changes in colon cancer progression are complex and might best be studied with more appropriate models. To that end, we (9) have recently reported on two normal cell lines which were derived from patients with colon cancer. Although they were verified by the pathologist as histologically normal areas of mucosa taken from the wide margins of surgical resection and are of epithelial origin, they display a subset of potentially premalignant features when grown in vitro. Thus, the continued development of additional normal colon mucosa (NCM) cell lines is still of great value.