In vitro propagation of Rivina humilis L. through proliferation of axillary shoots and shoot tips of mature plants

摘要

In vitro propagation of Rivina humilis L. was attempted through proliferation of axillary shoots and shoot tips obtained from mature plants. The shoot tips and nodal shoot segments exhibited 70 and 80% shoot initiation when cultured on Murashige and Skoog (MS) basal medium supplemented with 4.44 mu M 6-benzylaminopurine (BAP)+2.85 indole-3-acetic acid (IAA) and 8.88 mu M BAP+2.68 mu M naphthalene acetic acid (NAA), respectively. Shoots (2 to 3) were obtained from both shoot tip and nodal explants on MS medium supplemented with BAP (2.22 mu M), IAA (2.85 mu M) and silver nitrate (4 mu M AgNO3). Multiplication was maximum on MS medium supplemented with BAP (8.88 mu M), IAA (2.85 mu M) and AgNO3 (4 mu M) wherein 3-4 shoots per nodal explant were obtained. Microshoot elongation was achieved on MS medium containing BAP (2.22 mu M) and gibberellic acid (2.89 mu M GA(3)). Shoots (3-4 cm) exhibited 100% rooting within 4 wk in medium containing half MS with IAA (2.85-5.71 mu M) or IBA (2.45-4.90 mu M) alone or in combination with BAP (2.22 mu M), resulting in simultaneous rooting and shoot growth. About 70% of micropropagated plants were established successfully in micropots and transferred to the field after 3 months.