Production of Virus Free Plants from in Vitro Shoot Tips through in Vitro Meristem Culture

摘要

The present invention relates to: a method of producing an in vitro plant body by tissue-culturing a shoot tip for collecting a growing point for apical meristem culture; a proliferation medium for the method; a production method of virus and disease-free stocks through the apical meristem culture using the growing point collected from the in vitro plant body which is produced by the method; and a medium for the production method. According to the present invention, a user can collect the growing point without the limitation of the seasons, and mass-produce the plant bodies with low virus contamination rate and high survival rate, thereby contributing to the increase of income in farmhouses by easily supplying high quality varieties, and being expected to be able to export the high quality varieties by improving international competitiveness through enhancing the quality of products.