Separation of shrimp haemocytes (Penaeus monodon) and the opsonic effect of serum on phagocytic activity

摘要

Two cell types of shrimp haemocytes (Penaeus monodo;O were separated by continuous gradient centrifugation. Granular haemocytes and hyaline cells were demonstrated in vitro for their phagocytic activity using glutaraldehyde-fixed sheep red blood Cells (SRBC~f). The phagocytic activity of granular haemocytes was two times greater than that of hyaline cells. Haemocytes opsonized by shrimp serum increased phagocytic activity compared to the normal one, suggesting that opsonin in serum is one of the factors that play a role in the enhancement of shrimp phagocytosis.