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DEVELOPMENT OF GENE SPECIFIC PROBE AND PRIMERS FOR DETECTION OF INDIAN ISOLATE OF MOURILYAN VIRUS IN PENAEUS MONODON SHRIMP BY REAL TIME PCR
DEVELOPMENT OF GENE SPECIFIC PROBE AND PRIMERS FOR DETECTION OF INDIAN ISOLATE OF MOURILYAN VIRUS IN PENAEUS MONODON SHRIMP BY REAL TIME PCR
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机译:实时荧光定量PCR技术检测对虾对虾中印度病毒的印度分离株的基因特异性探针和探针的开发
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摘要
Based on the sequence information obtained for Mourilyan virus G2 protein gene of Indian isolate, a specific real time PCR primers and probe were developed. Mourilyan virus could be detected with increased sensitivity in infected shrimp tissues such as gills, gut, hepatopancreas, muscle and haemolymph. The real time analysis revealed highest Mourilyan virus copy numbers (22.5 x 105) µ-1 cDNA in gill tissue of shrimp, indicating gill tissues to be the appropriate sample for testing presence of MoV by real time PCR.
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机译:根据获得的印度分离株莫里里亚病毒G2蛋白基因序列信息,开发了特异性的实时PCR引物和探针。可以在感染的虾组织(如腮,肠,肝胰腺,肌肉和血淋巴)中以更高的灵敏度检测出毛里里亚病毒。实时分析显示,虾g组织中的毛里里亚病毒拷贝数最高(22.5 x 105)µ-1 cDNA,表明g组织是通过实时PCR检测MoV存在的合适样品。
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