Preparative separation of flavone dimers from        <i >Dysosma versipellisDysosma versipellis        by counter‐current chromatography: Trifluoroacetic acid as a solvent system modifier

Yang Zhi; Guo Peipei; Han Rui; Gao Jin‐Ming

摘要

Abstract > The separation of natural products is grueling and time‐consuming work with repeated isolations needed to obtain purified compounds. However, using counter‐current chromatography, a unique liquid–liquid partition chromatography, constituents can usually be purified efficiently. During the separation of flavone dimers from Dysosma versipellis (Hance) by counter‐current chromatography, the separation resolution and sample loading was impeded by the emulsification of the sample. By screening, trifluoroacetic acid was selected as the solvent modifier to eliminate the emulsification. Then, a quaternary solvent system of hexane/ethyl acetate/methanol/water (4:6:5:5 v/v/v/v) with trifluoroacetic acid at a low concentration of 0.5% v/v was used to purify the components from D. versipellis . Compared to that without trifluoroacetic acid, the separation resolution as well as the sample loading both increased greatly. In addition, flavone dimers in low concentrations could be enriched and purified at high sample loading. As a result, five podophyllotoxins and 11 flavonoids were purified and characterized by interpretation of spectroscopic data, in which two of eight flavone dimers were new and a known flavone dimer was first separated from this species. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >自然产品的分离是艰苦和耗时的工作，以获得净化所需的重复隔离化合物。然而，使用逆流色谱，通常可以有效地纯化独特的液体隔离层析，成分。通过逆流色谱法从脱蛋白葡萄球菌（Hance）分离黄酮二聚体（Hance），通过样品的乳化来阻抗分离分辨率和样品负载。通过筛选，选择三氟乙酸作为溶剂改性剂以消除乳化。然后，使用以低浓度为0.5％v / v的三氟乙酸的己烷/乙酸乙酯/甲醇/水（4：6：5：5V / v / v）的季溶剂系统用于纯化组分从 d。 versipellis </ i>。与没有三氟乙酸的情况相比，分离分辨率以及样品负荷两者都大大增加。此外，在高样品负载下可以富集和纯化低浓度的黄酮二聚体。结果，纯化了五种鼠毒素和11种异载物，其特征在于解释光谱数据，其中八个黄酮二聚体中的两个是新的，并且首先将已知的黄酮二聚体与该物种分离。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-35800/'>《Journal of separation science.》</a> <b style="margin: 0 2px;">|</b><span>2018年第18期</span><b style="margin: 0 2px;">|</b><span>共13页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yang Zhi&option=202" target="_blank" rel="nofollow">Yang Zhi;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guo Peipei&option=202" target="_blank" rel="nofollow">Guo Peipei;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Han Rui&option=202" target="_blank" rel="nofollow">Han Rui;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gao Jin‐Ming&option=202" target="_blank" rel="nofollow">Gao Jin‐Ming;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Shaanxi Key Laboratory of Natural Products and Chemical Biology College of Chemistry and PharmacyNorthwest A&</p> <p>F UniversityYangling P. R. China;</p> <p>Shaanxi Key Laboratory of Natural Products and Chemical Biology College of Chemistry and PharmacyNorthwest A&</p> <p>F UniversityYangling P. R. China;</p> <p>Shaanxi Key Laboratory of Natural Products and Chemical Biology College of Chemistry and PharmacyNorthwest A&</p> <p>F UniversityYangling P. R. China;</p> <p>Shaanxi Key Laboratory of Natural Products and Chemical Biology College of Chemistry and PharmacyNorthwest A&</p> <p>F UniversityYangling P. R. China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/159.html" title="化学">化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=counter‐current chromatography&option=203" rel="nofollow">counter‐current chromatography;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dysosma versipellis (Hance)&option=203" rel="nofollow">Dysosma versipellis (Hance);</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=flavone dimers&option=203" rel="nofollow">flavone dimers;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=traditional Chinese medicine&option=203" rel="nofollow">traditional Chinese medicine;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=trifluoroacetic acid&option=203" rel="nofollow">trifluoroacetic acid;</a> </p> <div class="translation"> 机译：逆流色谱;脱染症versipellis（Hance）;黄酮二聚体;中药;三氟乙酸; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025846462.html">Preparative separation of flavone dimers from <i >Dysosma 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Preparative separation of flavone dimers from Dysosma versipellisDysosma versipellis by counter‐current chromatography: Trifluoroacetic acid as a solvent system modifier

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