METHOD FOR PREPARING GINSENOSIDE Rg3 USING RECOMBINANT BETA-GLUCOSIDASE GENE-DERIVED ENZYME AND HIGH SPEED COUNTER CURRENT CHROMATOGRAPHY AFTER MEDIUM PRESSURE LIQUID CHROMATOGRAPHY PRETREATMENT

摘要

PURPOSE: A method for preparing ginsenoside Rg3 using recombinant beta-glucosidase gene-derived enzyme and high speed counter current chromatography (HSCCC) after medium pressure liquid chromatography (MPLC) pretreatment is provided to enhance the concentration and the purity of Rg3 in 1 ml of a red ginseng concentrate with a sugar content of 65 brix to approximately 280 mg and 75%, respectively.;CONSTITUTION: A method for effectively preparing a large amount of Rg3 from a red ginseng concentrate comprises the steps of: fermenting a red ginseng concentrate using enzyme produced from a recombinant beta-glucosidase gene; treating the red ginseng concentrate by microwaves for bioconversion of general ginsenoside into ginsenoside Rg3; and isolating and purifying the bioconverted Rg3 by MPLC and HSCCC. A strain which produces beta-glucosidase includes Bacillus subtilis, Paenibacillus polymyxa, Thermotoga neapolitana, Escherichia coli, Agrobacterium tumefaciens, Salmonella typhimurium, and Thermus caldophilus.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Sample: ginsenoside fraction from red ginseng extract (250mg)