H'/> Production of pure fractions of immunoglobulin G subclass autoantibodies against tumor necrosis factor
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Production of pure fractions of immunoglobulin G subclass autoantibodies against tumor necrosis factor

机译:免疫球蛋白G亚类自身抗体的纯分数对肿瘤坏死因子的生产

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Highlights ? A protocol for affinity purification of IgG subclass autoantibodies to TNF is proposed. ? Separation of IgG subclasses was performed using both positive and negative affinity chromatography. ? Ultrafiltration is used to remove impurities from TNF. ? Purification of IgG subclass anti-TNF autoantibodies will enable evaluation of their effect on TNF biological activity. Abstract Autoantibodies directed against cytokines are important effector molecules regulating the biological a
机译:<![cdata [ 突出显示 提出了对TNF的IgG子类自动逼真的亲和净化的协议。 < CE:列表项ID =“LSTI0010”> 使用正面和负关联的IgG子类分离色谱法。 超滤用于从TNF中移除杂质。 pu IgG亚类抗TNF自身抗体的resification将能够评估它们对TNF生物活性的影响。 抽象 针对细胞因子的自身抗体是调节生物A的重要效应分子

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