The recombinant plasmid DNA rFastBac-G2R-IgG, comprising a fragment of variola virus genome encoding the tumor necrosis factor binding protein, and fragments of the human genome the coding region of the immunoglobulin heavy chain G, and the strain of baculovirus BVG2RIgG, producing mouse soluble chimeric tumor necrosis factor binding protein variola virus SMALLPOX with a fragment of the heavy chain of immunoglobulin G MAN

摘要

FIELD: medicine.;SUBSTANCE: invention is related to preparation of protein, binding tumour necrosis factor (TNF), and may be used in medicine. Strain-producer of baculovirus BvG2RIgG is created with the help of recombinant plasmid DNA pFastBac-G2R-IgG with size of 6444 p.n. and molecular mass 4.18 mDa, which bears fragment of smallpox virus genome of strain India-1967, which codes protein that binds TNF, and fragment of human genome, which codes fragment of heavy chain of human antibody G. Produced strain produces soluble chimeric protein, which consists of smallpoz virus protein, which binds TNF, and fragment of heavy chain of human antibody G.;EFFECT: wider spectrum of new generation preparations intended for treatment of human diseases related to hyperproduction of tumour necrosis factor.;2 cl, 3 dwg, 1 tbl, 8 ex