Improving drought stress tolerance in fenugreek (Trigonella foenum-graecum) by exogenous melatonin

摘要

The present study was conducted to determine the changes which occurred in the water-stressed fenugreek (Trigonella foenum-gracum L.) in response to melatonin treatment. Fenugreek is a valuable medicinal plant which contains trigonelline that plays an osmoregulatory role in abiotic stress. To this end, the effects of three levels of water deficit in the first experiment and four concentrations of melatonin in the second experiment were investigated on the physiological and biochemical responses. The findings of the first experiment demonstrated that water deficit decreased the weight and length of fenugreek shoots as well as pigments contents, and catalase activity. In addition, such a deficit led to an increase in proline, trigonelline, melatonin and enhanced the activity of other antioxidant enzymes. Finally, the hydrogen peroxide and malondialdehyde concentrations increased under severe stress. The results of the second experiment indicated that melatonin treatment significantly reduced chlorophyll degradation. Furthermore, the activities of the ROS scavenging enzymes were increased by melatonin. Moreover, exogenous melatonin led to an increase in endogenous melatonin and trigonelline whereas it led to a decrease in proline content. These results revealed that fenugreek tolerated water deficit by increasing the endogenous melatonin and trigonelline, as well as the general physiological responses.