Activity of Neutral Phytase from Obesumbacterium proteus in Recombinant Strains of Yarrowia lipolytica under Cultivation on Low-Grade Vegetable Substrate

摘要

The characteristics of recombinant phytase from Obesumbacterium proteus have been studied after its integration into Yarrowia lipolytica with a Polf (pUV3-Op) plasmid. Phytase activity was observed in Y. lipolytica Polf transformants in a wide pH range (3.5-7.5) and remained at 80% of the initial value after 3-min heating at 95 degrees C, which made it possible to add the enzyme to a fodder produced with heat treatment. In the case of transformant cultivation on low-grade vegetable substrates, they also possessed confirmed phytase activity, as well as an increase in the content of intracellular phosphates as compared to cells of the wild-type Y. lipolytica strain.