Sperm cryopreservation and in vitro fertilisation in Zebrafish facilities at King's College London

摘要

King's College London (KCL) Zebrafish Facilities have the capacity to house 50,000 zebrafish (Danio rerio). There are currently 491 distinct lines of Zebrafish on the racks however, not all lines are actively and constantly in use. This created the need to establish an in-house Sperm Cryopreservation and In Vitro Fertilisation programme. At the time of writing, KCL has successfully frozen 94 lines.