The BIOne Benchtop Single-Use Bioreactor System For Mammalian Cell Growth And Recombinant Protein Production As A Robust Model For Bioprocess Development

摘要

Distek, Inc. has developed a benchtop scale single-use bioreactor (SUB) system for mammalian cell growth and recombinant protein production. The pre-sterilized BlOne system is engineered with a disposable headplate welded to a triple-layered liner that can be easily inserted into a non-sterile bioreactor glass vessel, converting it to a sterile, disposable SUB within a matter of seconds. The Distek BlOne system significantly reduces turnaround time by allowing users to seamlessly transition to a disposable platform while utilizing their existing capital equipment, without compromising the scalability of their current process. In this study, mammalian cultures were conducted by using either the Distek BlOne system or the traditional, non-disposable bioreactor system. Comparable cell culture performance was observed supporting that the Distek BlOne system provides a robust model for bioprocess development.